Altered Growth and Wood Characteristics in Transgenic Hybrid Aspen Expressing Agrobacterium tumefaciens T-DNA Indoleacetic Acid-Biosynthetic Genes.
Identifieur interne : 004B03 ( Main/Exploration ); précédent : 004B02; suivant : 004B04Altered Growth and Wood Characteristics in Transgenic Hybrid Aspen Expressing Agrobacterium tumefaciens T-DNA Indoleacetic Acid-Biosynthetic Genes.
Auteurs : H. Tuominen [Suède] ; F. Sitbon ; C. Jacobsson ; G. Sandberg ; O. Olsson ; B. SundbergSource :
- Plant physiology [ 1532-2548 ] ; 1995.
Abstract
A key regulator of cambial growth is the plant hormone indoleacetic acid (IAA). Here we report on altered wood characteristics and growth patterns in transgenic hybrid aspen (Populus tremula L. x Populus tremuloides Michx.) expressing Agrobacterium tumefaciens T-DNA IAA-biosynthetic iaaM and iaaH genes. Eighteen lines simultaneously expressing both genes were regenerated. Of these, four lines, verified to be transgenic by northern blot analysis, were selected and raised under controlled growth conditions. All four lines were affected in their growth patterns, including alterations in height and stem diameter growth, internode elongation, leaf enlargement, and degree of apical dominance. Two transgenic lines, showing the most distinct phenotypic deviation from the wild type, were characterized in more detail for free and conjugated IAA levels and for wood characteristics. Both lines showed an altered IAA balance, particularly in mature leaves and roots where IAA levels were elevated. They also exhibited changes in wood anatomy, most notably a reduction in vessel size, an increase in vessel density, and changes in ray development. Thus, the recent development of techniques for gene transfer to forest trees enabled us to investigate the influence of an altered IAA balance on xylem development in an intact experimental system. In addition, the results demonstrate the possibility of manipulating wood properties in a forest tree through controlled changes of IAA concentration and distribution.
DOI: 10.1104/pp.109.4.1179
PubMed: 12228661
PubMed Central: PMC157648
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Sitbon</name></author><author><name sortKey="Jacobsson, C" sort="Jacobsson, C" uniqKey="Jacobsson C" first="C." last="Jacobsson">C. Jacobsson</name></author><author><name sortKey="Sandberg, G" sort="Sandberg, G" uniqKey="Sandberg G" first="G." last="Sandberg">G. Sandberg</name></author><author><name sortKey="Olsson, O" sort="Olsson, O" uniqKey="Olsson O" first="O." last="Olsson">O. Olsson</name></author><author><name sortKey="Sundberg, B" sort="Sundberg, B" uniqKey="Sundberg B" first="B." last="Sundberg">B. Sundberg</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1995">1995</date><idno type="RBID">pubmed:12228661</idno><idno type="pmid">12228661</idno><idno type="pmc">PMC157648</idno><idno type="doi">10.1104/pp.109.4.1179</idno><idno type="wicri:Area/Main/Corpus">004A64</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004A64</idno><idno type="wicri:Area/Main/Curation">004A64</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004A64</idno><idno type="wicri:Area/Main/Exploration">004A64</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Altered Growth and Wood Characteristics in Transgenic Hybrid Aspen Expressing Agrobacterium tumefaciens T-DNA Indoleacetic Acid-Biosynthetic Genes.</title><author><name sortKey="Tuominen, H" sort="Tuominen, H" uniqKey="Tuominen H" first="H." last="Tuominen">H. Tuominen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Forest Genetics and Plant Physiology, The Swedish University of Agricultural Sciences, S-90183 Umea, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Forest Genetics and Plant Physiology, The Swedish University of Agricultural Sciences, S-90183 Umea</wicri:regionArea><wicri:noRegion>S-90183 Umea</wicri:noRegion></affiliation></author><author><name sortKey="Sitbon, F" sort="Sitbon, F" uniqKey="Sitbon F" first="F." last="Sitbon">F. Sitbon</name></author><author><name sortKey="Jacobsson, C" sort="Jacobsson, C" uniqKey="Jacobsson C" first="C." last="Jacobsson">C. Jacobsson</name></author><author><name sortKey="Sandberg, G" sort="Sandberg, G" uniqKey="Sandberg G" first="G." last="Sandberg">G. Sandberg</name></author><author><name sortKey="Olsson, O" sort="Olsson, O" uniqKey="Olsson O" first="O." last="Olsson">O. Olsson</name></author><author><name sortKey="Sundberg, B" sort="Sundberg, B" uniqKey="Sundberg B" first="B." last="Sundberg">B. Sundberg</name></author></analytic><series><title level="j">Plant physiology</title><idno type="eISSN">1532-2548</idno><imprint><date when="1995" type="published">1995</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A key regulator of cambial growth is the plant hormone indoleacetic acid (IAA). Here we report on altered wood characteristics and growth patterns in transgenic hybrid aspen (Populus tremula L. x Populus tremuloides Michx.) expressing Agrobacterium tumefaciens T-DNA IAA-biosynthetic iaaM and iaaH genes. Eighteen lines simultaneously expressing both genes were regenerated. Of these, four lines, verified to be transgenic by northern blot analysis, were selected and raised under controlled growth conditions. All four lines were affected in their growth patterns, including alterations in height and stem diameter growth, internode elongation, leaf enlargement, and degree of apical dominance. Two transgenic lines, showing the most distinct phenotypic deviation from the wild type, were characterized in more detail for free and conjugated IAA levels and for wood characteristics. Both lines showed an altered IAA balance, particularly in mature leaves and roots where IAA levels were elevated. They also exhibited changes in wood anatomy, most notably a reduction in vessel size, an increase in vessel density, and changes in ray development. Thus, the recent development of techniques for gene transfer to forest trees enabled us to investigate the influence of an altered IAA balance on xylem development in an intact experimental system. In addition, the results demonstrate the possibility of manipulating wood properties in a forest tree through controlled changes of IAA concentration and distribution.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">12228661</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1532-2548</ISSN><JournalIssue CitedMedium="Internet"><Volume>109</Volume><Issue>4</Issue><PubDate><Year>1995</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Altered Growth and Wood Characteristics in Transgenic Hybrid Aspen Expressing Agrobacterium tumefaciens T-DNA Indoleacetic Acid-Biosynthetic Genes.</ArticleTitle><Pagination><MedlinePgn>1179-1189</MedlinePgn></Pagination><Abstract><AbstractText>A key regulator of cambial growth is the plant hormone indoleacetic acid (IAA). Here we report on altered wood characteristics and growth patterns in transgenic hybrid aspen (Populus tremula L. x Populus tremuloides Michx.) expressing Agrobacterium tumefaciens T-DNA IAA-biosynthetic iaaM and iaaH genes. Eighteen lines simultaneously expressing both genes were regenerated. Of these, four lines, verified to be transgenic by northern blot analysis, were selected and raised under controlled growth conditions. All four lines were affected in their growth patterns, including alterations in height and stem diameter growth, internode elongation, leaf enlargement, and degree of apical dominance. Two transgenic lines, showing the most distinct phenotypic deviation from the wild type, were characterized in more detail for free and conjugated IAA levels and for wood characteristics. Both lines showed an altered IAA balance, particularly in mature leaves and roots where IAA levels were elevated. They also exhibited changes in wood anatomy, most notably a reduction in vessel size, an increase in vessel density, and changes in ray development. Thus, the recent development of techniques for gene transfer to forest trees enabled us to investigate the influence of an altered IAA balance on xylem development in an intact experimental system. In addition, the results demonstrate the possibility of manipulating wood properties in a forest tree through controlled changes of IAA concentration and distribution.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tuominen</LastName><ForeName>H.</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Forest Genetics and Plant Physiology, The Swedish University of Agricultural Sciences, S-90183 Umea, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sitbon</LastName><ForeName>F.</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Jacobsson</LastName><ForeName>C.</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Sandberg</LastName><ForeName>G.</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Olsson</LastName><ForeName>O.</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Sundberg</LastName><ForeName>B.</ForeName><Initials>B</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1995</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>9</Month><Day>14</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1995</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12228661</ArticleId><ArticleId IdType="pii">109/4/1179</ArticleId><ArticleId IdType="pmc">PMC157648</ArticleId><ArticleId IdType="doi">10.1104/pp.109.4.1179</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Mol Gen Genet. 1991 Dec;230(3):463-74</Citation><ArticleIdList><ArticleId IdType="pubmed">1766442</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1990 Sep;15(3):373-81</Citation><ArticleIdList><ArticleId IdType="pubmed">2103458</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1987 Jan;84(1):131-5</Citation><ArticleIdList><ArticleId IdType="pubmed">16593793</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1989 Nov;86(21):8467-71</Citation><ArticleIdList><ArticleId IdType="pubmed">2554318</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1989 May;86(9):3219-23</Citation><ArticleIdList><ArticleId IdType="pubmed">16594033</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1991 Feb;95(2):480-5</Citation><ArticleIdList><ArticleId IdType="pubmed">16668009</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1986 Feb 7;231(4738):616-8</Citation><ArticleIdList><ArticleId IdType="pubmed">3511528</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1993 Jul;102(3):1033-1036</Citation><ArticleIdList><ArticleId IdType="pubmed">12231885</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Biochem. 1984 Jan 16;138(2):387-91</Citation><ArticleIdList><ArticleId IdType="pubmed">6365544</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1990 Dec;94(4):1721-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16667908</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 1984 Dec 1;3(12):2723-30</Citation><ArticleIdList><ArticleId IdType="pubmed">16453574</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1984 Aug;81(16):5071-5</Citation><ArticleIdList><ArticleId IdType="pubmed">6089175</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 1993 Sep;4(3):495-505</Citation><ArticleIdList><ArticleId IdType="pubmed">8220492</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Jacobsson, C" sort="Jacobsson, C" uniqKey="Jacobsson C" first="C." last="Jacobsson">C. Jacobsson</name><name sortKey="Olsson, O" sort="Olsson, O" uniqKey="Olsson O" first="O." last="Olsson">O. Olsson</name><name sortKey="Sandberg, G" sort="Sandberg, G" uniqKey="Sandberg G" first="G." last="Sandberg">G. Sandberg</name><name sortKey="Sitbon, F" sort="Sitbon, F" uniqKey="Sitbon F" first="F." last="Sitbon">F. Sitbon</name><name sortKey="Sundberg, B" sort="Sundberg, B" uniqKey="Sundberg B" first="B." last="Sundberg">B. Sundberg</name></noCountry><country name="Suède"><noRegion><name sortKey="Tuominen, H" sort="Tuominen, H" uniqKey="Tuominen H" first="H." last="Tuominen">H. Tuominen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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